A survey of physical methods used to examine the structure and function of biological macromolecules. 
 
 

Instructor:	Dr. Michael Henzl
Office:	11C Schlundt Annex
Office Hours:	By appointment.
Prerequisites:	Physical Chemistry, Calculus, Introductory Biochemistry
Grading:	Exams (20%), Classroom participation (20%), Assignments (60%)
Resources:
Textbook:	Principles of Physical Biochemistry by Van Holde, Johnson, and Ho. 2nd Edition. Prentice-Hall, 2006.
Additional reading materials will be placed near the copy machine in Schweitzer Hall.
Additional Resources:	The following additional resources are available at Ellis library:
	Biological physics (D. C. S. White)
	Biothermodynamics: The Study of Biochemical Processes at Equilibrium (J.T. Edsall and H. Gutfreund)
	Physical Chemistry of Macromolecules (C. Tanford)
	The Structure and Properties of Water (D. Eisenberg and W. Kauzmann)
	Biophysical Chemistry, vols. 1-3 (C.R. Cantor, P.R. Schimmel)
	Binding and Linkage: Functional Chemistry of Biological Macromolecules (J. Wyman and S.J. Gill)
	Protein Interactions (G. Weber)
	Physical Biochemistry: Applications to Biochemistry and Molecular Biology (D. Freifelder)
	Physical Chemistry for Students of Biology and Chemistry (D. Freifelder)
	Principles and Problems in Physical Chemistry for Biochemists (N.C. Price and R.A. Dwek)
	Biophysical Chemistry: Principles, Techniques, and Applications (A.G. Marshall)

Disabilities: 
If you need accommodations because of a disability, if you have emergency medical information to share with me, or if you need special arrangements in case the building must be evacuated, please inform me immediately. Please see me privately after class, or at my office. 
 
 

Class No.	Date	Topic
1	Jan. 17	Discussion of syllabus, Spectroscopy (light scattering)
2	Jan. 19	Spectroscopy (light scattering)
3	Jan. 22	Spectroscopy (light scattering)
4	Jan. 24	Spectroscopy (absorption)
5	Jan. 26	Spectroscopy (absorption)
6	Jan. 29	Spectroscopy (Raman scattering)
7	Jan.31	Spectroscopy (circular dichroism)
8	Feb. 2	Spectroscopy (fluorescence)
9	Feb. 5	Spectroscopy (fluorescence)
10	Feb. 7	Spectroscopy (fluorescence)
11	Feb. 9	Spectroscopy (fluorescence)
12	Feb. 12	Thermodynamics (Review)
13	Feb. 14	Thermodynamics (Review)
14	Feb. 16	Thermodynamics (Introduction to statistical thermodynamics)
15	Feb. 19	Thermodynamics (Introduction to statistical thermodynamics)
16	Feb. 21	Thermodynamics (ligand binding)
17	Feb. 23	Thermodynamics (ligand binding)
18	Feb. 26	Thermodynamics (ligand binding)
19	Feb. 28	Thermodynamics (ligand binding)
20	Mar. 2	Thermodynamics (macromolecular stability)
21	Mar. 5	Thermodynamics (macromolecular stability)
22	Mar. 7	Thermodynamics (macromolecular stability)
23	Mar. 9	Molecular Thermodynamics (Tanner)
24	Mar. 12	Molecular Thermodynamics (Tanner)
25	Mar. 14	Viscosity
26	Mar. 16	Solution thermodynamics (Gibbs-Duhem Eqn; ideal/nonideal solutions)
-	Mar. 19	Solution thermodynamics (dialysis, osmotic pressure)
-	Mar. 21	Solution thermodynamics (sedimentation equilibrium)
-	Mar. 23	Solution thermodynamics (sedimentation equilibrium)
27	Mar. 26	Spring Recess
28	Mar. 28	Spring Recess
29	Mar. 28	Spring Recess
30	April 2	Transport Processes (diffusion)
31	April 4	Transport Processes (diffusion)
32	April 6	Transport Processes (electrophoresis)
33	April 9	Transport Processes (sedimentation velocity)
34	April 11	Transport Processes (sedimentation velocity)
35	April 13	Nuclear Magnetic Resonance (Van Doren)
36	April 16	Nuclear Magnetic Resonance (Van Doren)
37	April 18	Nuclear Magnetic Resonance (Van Doren)
38	April 20	Nuclear Magnetic Resonance (Van Doren)
39	April 23	Nuclear Magnetic Resonance (Van Doren)
40	April 25	X-ray Crystallography (Beamer)
41	April 27	X-ray Crystallography (Beamer)
42	April 30	X-ray Crystallography (Beamer)
43	May 2	X-ray Crystallography (Beamer)
44	May 4	Hour Exam (NMR, x-ray crystallography)