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After equilibrating at temperature, the cell was accelerated to 4000 rpm. The reference and sample chambers in the synthetic-boundary cell are connected by a very narrow channel. The centrifugal force generated as the sample spins drives solvent into the sample compartment until the liquid column heights are equalized. This has the effect of gently layering pure solvent over the sample, creating a sample-buffer interface. The decay of the interface, due to diffusion, is then monitored with time. Analyze the data contained in the five spreadsheets to obtain an estimate of the diffusion coefficient for this protein. General procedure: Fit each data set to sigmoidal function (e.g., sigmoidal Richards function type 2). Simulate data with optimized parameters. After fitting all five data sets, determine dc/dr values at the boundary by. Solve for D. @ ResultsLog     6 4^BA:WbBA  2008_diffusion_project                    %  (